![]() Rabbit monoclonal antibodies have overcome this limitation, providing access to defined reagents of infinite supply from the rabbit antibody repertoire. As a consequence, epitopes conserved between rodent and human antigens that are invisible to rodent monoclonal antibodies (and also human monoclonal antibodies generated from transgenic mice with human immunoglobulin genes) can often be recognized by rabbit antibodies. In addition, rabbits, which belong to the order Lagomorpha (lagomorphs), are evolutionarily distant from mice and rats, which belong to the order Rodentia (rodents). The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies and monoclonal antibodies that combine high specificity with high avidity and affinity. ![]() Chimeric rabbit/human Fab libraries are made in our pC Display phagemid vectors by fusing rabbit V κ/V λ to human C κ and rabbit V H to human CH1 of human IgG 1. cDNA from spleen and bone marrow samples derived from multiple groups of animals immunized by different antigens can be pooled to make a unified library for all the antigens. Total RNA from spleen lymphocytes and bone marrow cells are purified, and then the first-strand cDNAs are synthesized. Usually, two or more rabbits of the b9 allotype are immunized with one antigen. We generate rabbit antibody libraries and select high affinity antigen-specific antibodies by phage display following Dr. In contrast to rabbit hybridoma technology available in other leading companies, Creative Biolabs has built up a unique rabbit monoclonal antibody production platform based on our unparalleled expertise in phage display antibody library technology. HighlightsĬonstruction of Immunized Rabbit Antibody Library With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects. Cell-based Membrane Protein Expression and PurificationĬreative Biolabs is offering the most comprehensive services for antibody development projects. ![]() Cell-free Membrane Protein Expression and Purification.Membrane Protein Solubilization and Stabilization.Manufacturability Assessment and Optimization.SIAT ® Immunogenicity Assessment Services.CreMap™ Epitope Mapping & Discovery Services.Dual-Polarization Interferometry (DPI) Services.Differential Scanning Calorimetry (DSC) Services.Isothermal Titration Calorimetry (ITC) Services.Magic™ Human Antibody Production Using Transgenic Mice.Bovine Ultralong CDR3 Antibody Humanization.Antibody Off-target Identification and Specificity Profiling Service.Biomolecular Interaction Analysis Services.Epitope Prediction for the Identification of Antibody Epitopes.Epitope Mapping by X-ray Crystallography.Phage Display & Antibody Library Services.
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